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. 2020 Jul 9;5(13):e137289. doi: 10.1172/jci.insight.137289

Figure 1. RAGE is expressed in regressing atherosclerotic plaques.

Figure 1

(A) Model schematic: atherosclerotic aortic arches from Ldlr—/— mice were transplanted into WT, Ager—/—, diaphanous related formin 1–deficient (Diaph1—/—), or Tg glyoxalase-1 (Glo1) recipient mice and harvested at the indicated times after aortic arch transplantation. (B) Representative images of en face Sudan IV staining of atherosclerotic lesions in nondiabetic Ldlr—/—and diabetic Ldlr—/— mice. Quantification of plaque area as percentage of total aortic surface area is shown; N = 10 mice/group. (C) Immunofluorescence staining for RAGE and CD68 of atherosclerotic plaques 5 days after aortic arch transplantation. Representative images from N = 4 mice/group are shown. Scale bars: 250 μm, and inset, 50 μm. The secondary antibody–alone control is shown. (D) Laser capture microdissection of CD68+ cells 5 days posttransplantation. Representative images from N = 4 mice/group are shown. Scale bar: 100 μm. (E) Ager mRNA expression in macrophages captured from D (N = 4 mice/group). Mean ± SEM. Unpaired t test was performed in B and E. ****P < 0.0001.