Figure 7. Deficiency of myeloid cell podoplanin increases complement activation in early sepsis.

WT and LysM Pdpn^–/– mice were i.p. injected with a half-lethal dose of E. coli (2 × 10⁷ CFU/mouse). Peripheral blood was collected from mice without E. coli injection or at different time points after E. coli infection. The complement activation was determined by measuring the plasma levels of C5a (A), C3a (B), and C5b-9 (C) with ELISAs (n = 9 mice/group). The data were presented as mean ± SD. ^#P < 0.05, comparing the sample at 0 hours of E. coli injection with samples at other time points after E. coli infection. *P < 0.05. **P < 0.01. Data were analyzed using 1-way ANOVA followed by Tukey’s test for multiple groups and were representative of 3 independent experiments.