Figure 1.

a, Representative immunoblot and quantification of CnA and CnB expression in SCs isolated from sciatic nerves of WT and CnB^scko pups, normalized to GAPDH, and reported relative to the WT expression level. All pups of the same genotype from one litter (2-4 mice) were pooled for a single experiment, which represents one WB band. This was repeated 3 times to generate n = 3. Error bar indicates SEM. ****p < 0.0001 (Student's t test). b, Semithin images of sciatic nerves from P1, P10, P28, and 6-month-old WT and CnB^scko mice. Scale bar, 10 µm. c, Quantification of myelin thickness measured by g-ratio (axon diameter/fiber diameter) from EM images. N = 3 mice/genotype. Error bar indicates SEM. d, Representative WB images of MAG, P0, PMP22, MBP, KROX20, and GAPDH in sciatic nerve from WT and CnB^scko mice aged P1, P10, P28, and 6 months. PMP22 and MBP could not be detected in the WBs from P1 animals. e, Quantification of WBs in d for P1, P10, P28, and 6 months WT and CnB^scko nerves. Protein levels were normalized to GAPDH and reported compared with WT. N = 4 pooled groups (P1) (3 or 4 mice per group), n = 10 mice (P10), n = 9 mice (P28), n = 4 mice (6 months) f, EM images of bundles with unsorted axons (*) and amyelinated fibers (arrows) from P1, P10, P28, and 6 months old CnB^scko and WT mice. Scale bar, 2 μm. g, Quantification of the percentage of total bundles counted from 20 to 30 EM images with unsorted axons in CnB^scko and WT mice at P1, 10, P28, and 6 months. Error bar indicates SEM. **p < 0.01, *p < 0.05. h, Quantification of the number of amyelinated axons per whole sciatic nerve cross section in P1, P10, P28, and 6 months CnB^scko and WT mice. Error bar indicates SEM. **p < 0.01, ***p < 0.001.