Figure 3.

a, Representative images of WT and CnB^scko sciatic nerves 7 d after nerve crush. Red boxes represent intact myelin sheaths. Scale bars, 20 µm. b, Quantification of the number of intact myelin sheaths (a, red boxes) per whole nerve cross section of WT and CnB^scko mice 7 d after sciatic nerve crush. N = 3 mice/genotype. Error bars indicate SEM. *p < 0.05 (Student's t test). c, Representative images of YFP-labeled axons distal to the crush site of WT and CnB^scko mice at 3 dpc. Scale bar, 100 µm. d, Quantification of YFP-labeled axons in c. The axon fragments in each image were measured and averaged, three 40× images per animal were obtained, and the final number reported for each animal was an average of the three images. N = 5 or 6 mice. Error bar indicates SEM. **p < 0.01. e, MCP-1 mRNA expression in the distal portion of WT and CnB^scko nerves at 3 dpc. Expression reported relative to average WT expression. N = 3 mice/genotype. Error bar indicates SEM. f, Representative images from longitudinal sections of the distal stump of crushed WT and CnB^scko sciatic nerves at 3 and 7 dpc. Macrophages are stained in red by F480. Scale bar, 50 µm. g, Quantification of the macrophages from the longitudinal sections in f. N = 5 mice/genotype for 3 dpc and N = 4 mice/genotype for 7 dpc. Error bar indicates SEM.