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. 2020 Aug 5;40(32):6165–6176. doi: 10.1523/JNEUROSCI.0951-20.2020

Figure 4.

Figure 4.

a, Reconstructed cross sections and high-powered representative images from sciatic nerve ex vivo stained for myelin basic protein (MBP) and neurofilament (NF). Scale bars: cross section, 200 µm; high-powered image, 20 µm. b, Quantification of the number of intact axons and MBP rings remaining in a cross section of WT and CnBscko sciatic nerves after 12 d ex vivo (12 DEV). N = 4 animals/genotype. Error bar indicates SEM. **p < 0.01; ***p < 0.001; Student's t test. c, Representative images of primary mouse SCs isolated from the sciatic nerves of P10 WT and CnBscko pups, stained for S100 (SC) and P0. Scale bar, 2 µm. d, Quantification of the percentage of SCs (marked by S100 staining) still containing myelin protein P0 after 7 d in culture. N = 4 coverslips/genotype per time point. Each coverslip contained SCs from 1 individual animal. Error bar indicates SEM. Two-way ANOVA: ****p < 0.0001 (WT 1 DIV vs 7 DIV); ***p < 0.001 (CnB 1 DIV vs 7 DIV); ###p < 0.001 (WT 7 DIV vs CnB 7 DIV).