Figure 2.

CRISPR/Cas9-generated cdnf mutant zebrafish. A, Scheme of CRISPR/Cas9-generated 14-base pair deletion in exon2 of cdnf. B, Sequence chromatogram showing a premature stop codon of a homozygous cdnf mutant zebrafish. C, Results of the HRM analysis showing the distinctive melting curves of each genotype. D, Reduction in cdnf mRNA expression shown in the brains of 8 dpf and E, 1 mpf cdnf mutant fish. F, No obvious gross phenotype in larvae and adult cdnf mutant fish. PPa, anterior parvocellular preoptic nucleus; T, midbrain tegmentum; TeO, tectum opticum. Scale bars: D, 200 µm; F, 10 dpf, 1 mm; 10 mpf, 1 cm.