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. 2020 Aug 5;40(32):6146–6164. doi: 10.1523/JNEUROSCI.2636-19.2020

Figure 3.

Figure 3.

qRT-PCR analysis of cdnf and neurotransmitter synthesis enzymes at larval, adulthood, and aging stages. A–C, Significant reduction of cdnf mRNA in cdnf-deficient (KO) fish (unpaired t test, t = 4.675, df = 22, two-tailed p = 0.0001; t = 4.245, df = 8, p = 0.0028; t = 3.280, df = 8, p = 0.0112). D–F, Significant increase of th2 transcripts in cdnf-deficient fish (unpaired t test, t = 4.043, df = 22, p = 0.0005; t = 2.901, df = 8, p = 0.0198; t = 5.450, df = 8, p = 0.0006). G–I, Nonsignificant differences in th1 mRNA expression between groups (unpaired t test, t = 0.6318, df = 22, p = 0.5340; t = 1.024, df = 8, p = 0.3358; t = 0.7477, df = 8, p = 0.4760). J–L, Significant reduction of hdc mRNA expression in adult brains (unpaired t test, t = 0.052, df = 22, p = 0.9590; t = 2.473, df = 8, p = 0.0385; t = 1.042, df = 8, p = 0.3278). qRT-PCR analysis relative to expression of the housekeeping gene rpl13a. Values are mean ± SEM; n = 12/group for 8 dpf fish, n = 5/group for 8 mpf brains, n = 5/group for 18 mpf brains in each cdnf KO and WT group. Data are mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; Student's t test.