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. 2020 Aug 5;40(32):6146–6164. doi: 10.1523/JNEUROSCI.2636-19.2020

Figure 7.

Figure 7.

slc32a/vGAT downregulation in cdnf KO fish. A, Quantification of slc32a/vGAT mRNA expression relative to the housekeeping gene rpl13a in 8 dpf fish (unpaired t test, t = 2.508, df = 14, p = 0.0251, n = 8/group). B, ISH of vGAT mRNA expression of 8 dpf brains (n = 5). C, Quantification of gad1b mRNA expression relative to rpl13a in 8 dpf fish (unpaired t test, t = 2.077, df = 14, p = 0.0567). D, Quantification of gad2a mRNA expression relative to the rpl13a in 8 dpf fish (unpaired t test, t = 2.508, df = 14, p = 0.0251). E, Quantification of vGlut2 mRNA expression relative to rpl13a in 8 dpf fish (unpaired t test, t = 2.508, df = 14, p = 0.0251). Arrows and red rectangles represent downregulated expression of vGAT in the hypothalamus (H), preoptic region (Po), ventral posterior tuberculum (PTv), and ventral thalamus (VT). Data are mean ± SEM. *p < 0.05 (Student's t test). Scale bar, 100 µm.