Table 2.

HPLC analysis of monoamine concentrations of larvae, adult brains, and aging brains^a

	19-month-old brain (pmol/mg protein)			8-month-old brain (pmol/mg protein)			8 dpf (8 larvae per tube) (pmol/mg protein)
	WT (n = 5)	KO (n = 5)	p	WT (n = 9)	KO (n = 9)	p	WT (n = 3)	KO (n = 3)	p
Dopamine	22.39 ± 0.3935	21.14 ± 0.6298	0.13	16.52 ± 0.542	15.97 ± 0.433	0.4451	2.834 ± 0.1438	3.21 ± 0.2845	0.3041
Norepinephrine	58.79 ± 3.076	56.32 ± 2.85	0.5707	46.29 ± 1.956	43.3 ± 1.243	0.216	7.2 ± 0.1838	8.239 ± 0.56	0.1528
DOPAC	4.699 ± 0.3663	3.506 ± 0.2053b	0.0218	2.764 ± 0.1298	2.452 ± 0.2412	0.2713	1.721 ± 0.1544	1.605 ± 0.08641	0.549
Homovanillic acid	12.48 ± 1.131	8.847 ± 0.1623b	0.013	17.54 ± 1.768	8.494 ± 1.607b	0.0016	2.235 ± 0.007714	4.195 ± 1.205	0.1794
3-Methoxytyramine	1.539 ± 0.1343	1.343 ± 0.2165	0.4639	5.42 ± 0.6011	5.795 ± 0.438	0.6205	2.854 ± 0.1544	3.351 ± 0.4868	0.3855
Serotonin	23 ± 1.162	24.63 ± 0.6439	0.2543	16.83 ± 0.6696	19.43 ± 0.6398c	0.0124	3.693 ± 0.157	3.902 ± 0.1672	0.4142
5-Hydroxyindoleacetic acid	19.81 ± 0.9596	13.7 ± 0.4645b	0.0004	15.99 ± 0.8659	15.08 ± 1.173	0.5423	3.769 ± 0.03767	5.02 ± 0.6834	0.1416
Histamine	9.065 ± 0.4468	11.33 ± 1.745	0.244	5.958 ± 0.516	4.285 ± 0.3379b	0.0154	7.805 ± 0.854	5.899 ± 1.09	0.2406

^aData are mean ± SEM. Student's t test was used for statistical analysis.

^bA significant reduction in KO.

^cA significant increase in KO.