Figure 1. MSCs direct differentiation of BM cells into CD11b^midLy6C^midLy6G^lo cells under inflammatory stimulation.

BM cells extracted from C57BL/6 mice were cocultured with MSCs in direct coculture or Transwell system under GM-CSF stimulation (40 ng/mL) for 5 days and assayed. After gating BM cells on Ly6G, Ly6G^lo cells were assessed for CD11b and Ly6C expression by flow cytometry. Representative cytograms and the percentages of CD11b^loLy6C^loLy6G^lo cells, CD11b^midLy6C^midLy6G^lo cells, and CD11b^hiLy6C^hiLy6G^lo cells of total BM cells are presented. Data (mean ± SD) are from 4 independent sets of experiments (n = 4 in each group per set). A dot depicts data from 1 biological sample. ***P < 0.001, ****P < 0.0001 by 1-way ANOVA and Tukey’s multiple-comparison test.