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. 2020 Jun 18;5(12):e138345. doi: 10.1172/jci.insight.138345

Figure 2. The chemokine pathway detected by urine proteomics reflects intrarenal production by myeloid, CD8+, and NK cells.

Figure 2

(A) Uniform manifold approximation and projection (UMAP) plot of single-cell RNA-Seq of 24 renal biopsies of patients with active lupus nephritis (medium resolution clustering). (B) The identity of the cell clusters was determined by the expression of lineage markers. (C–F) A chemokine and an IFN-γ response score based on the expression of the markers in the top urine PC1 pathways (“chemokine-mediated signaling pathway,” GO:0070098; and “cellular response to interferon-gamma,” GO:0071346)) identified kidney-infiltrating myeloid, CD8+, and NK cells as the main producers of the chemokines in response to IFN-γ detected in the urine. (G) Distribution of IFN-γ–, TNF-, and IL-1β–expressing cells. (H) Distribution of the total unique molecular identifiers (UMIs) mapped to IFN-γ, TNF, and IL-1β as a proxy for the amount of cytokine produced by each cell type.