Skip to main content
. 2020 Jun 18;5(12):e136720. doi: 10.1172/jci.insight.136720

Figure 5. IRF1 regulates PANoptosis.

Figure 5

(A) Representative images of TUNEL staining of colons from azoxymethane/dextran sulfate sodium–treated (AOM/DSS-treated) WT and Irf1–/– mice on days 0, 14, and 80. (B) Representative images of cleaved caspase-3 (CASP3) staining of colon tissues from DSS-treated WT and Irf1–/– mice on days 0 and 14 after AOM injection. Scale bar: 100 μM. (C) Immunoblot analysis of the pro- and cleaved forms of CASP3 and caspase-7 (CASP7) in colons of WT and Irf1–/– mice on days 0, 14, and 80 after AOM injection. Blots represent data from the same biological samples at the indicated time point run in parallel. (D) Immunoblot analysis of GSDMD and MLKL in colons of WT and Irf1–/– mice 14 days after AOM injection. Blots represent data from the same biological samples run in parallel. (E) Cell death analysis of intestinal organoids derived from WT and Irf1–/– mice after stimulation with TNF + zVAD. **P < 0.01; ***P < 0.001. The 2-tailed t test (E) was used. Data are representative of 3 independent experiments. Data are represented as mean ± SEM.