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. 2020 Jul 31;9(7):bio053132. doi: 10.1242/bio.053132

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Quantification of transient amplifying cells and neuroblasts within the SGZ of Slc13a4^+/+ and Slc13a4^+/− brains at 12 weeks. (A) Representative lower magnification image from a wild-type SGZ labelled with DCX and TBR2, and higher magnification images labelled with (B) DCX, (C) TBR2, (D) merge. (E) A representative lower magnification image from a Slc13a4^+/− SGZ labelled with DCX and TBR2, and higher magnification images labelled with (F) DCX, (G) TBR2 and (H) merge. (I) An increase in the number of transient amplifying cells (TBR2⁺) were observed in Slc13a4^+/− SGZ compared with wild-type controls. (J) No difference in the number of DCX⁺ neuroblasts was observed in Slc13a4^+/− SGZ compared with wild-type controls. n=3 for each genotype. Mean±s.e., Student's t-test, *P<0.05. Arrows highlight TBR2⁺ cells. Scale bar: 200 µm in A and E, 25 µm in B, C, D, F, G and H.