Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | E. coli K-12 BW25113 | Yale Coli Genetic Stock Center | CGSC#: 7636 | |
| Strain, strain background (Escherichia coli) | BW25113 ΔnuoA | Dharmacon Keio | OEC4987-213603796 | |
| Strain, strain background (Escherichia coli) | BW25113 ΔnuoB | Dharmacon Keio | OEC4987-213603795 | |
| Strain, strain background (Escherichia coli) | BW25113 ΔnuoH | Dharmacon Keio | OEC4987-213603791 | |
| Strain, strain background (Escherichia coli) | BW25113 ΔnuoI | Dharmacon Keio | OEC4987-213603790 | |
| Strain, strain background (Escherichia coli) | BW25113 ΔatpA | Dharmacon Keio | OEC4987-213606163 | |
| Strain, strain background (Escherichia coli) | BW25113 ΔatpB | Dharmacon Keio | OEC4987-213606167 | |
| Strain, strain background (Escherichia coli) | BW25113 ΔatpC | Dharmacon Keio | OEC4987-213605824 | |
| Strain, strain background (Escherichia coli) | BW25113 ΔatpD | Dharmacon Keio | OEC4987-213605825 | |
| Strain, strain background (Escherichia coli) | BW25113 ΔatpE | Dharmacon Keio | OEC4987-213606166 | |
| Strain, strain background (Escherichia coli) | BW25113 ΔatpF | Dharmacon Keio | OEC4987-213606165 | |
| Strain, strain background (Escherichia coli) | BW25113 ΔatpG | Dharmacon Keio | OEC4987-213607977 | |
| Strain, strain background (Escherichia coli) | BW25113 ΔatpH | Dharmacon Keio | OEC4987-213606164 | |
| Strain, strain background (Escherichia coli) | E. coli DK8 1100∆(uncB-uncC)ilv::TnlO | Rubinstein lab (created in Guo et al., 2019) | DK8 | |
| Strain, strain background (Escherichia coli) | BL21(DE3) | Sigma-Aldrich | CMC0016 | Electrocompetent cells |
| Transfected construct (bacterial) | Plasmid: pKL09-GCaMP6f-mScarlet bb118 | This paper | Addgene #pending | GCaMP6-mScarlet |
| Transfected construct (bacterial) | Plasmid: pKL10-mRuby-iATPSnFR1.0 bb118 | This paper | Addgene # pending | mRuby-ATPSnfr |
| Transfected construct (bacterial) | Plasmid: pKL12-GCaMP pHuji bb118 | This paper | Addgene # pending | GCaMP6-pHuji |
| Transfected construct (bacterial) | Plasmid: pKL13-MgtC bb118 | This paper | Addgene # pending | S. typhimurium mgtC expression plasmid |
| Transfected construct (bacterial) | Plasmid: pKL11-GCaMP6f bb100 | This paper | Addgene # pending | GCaAMP6 |
| Chemical compound, drug | Glucose | Sigma | G7528-1KG | |
| Chemical compound, drug | M9 salts | Sigma | M6030 | |
| Chemical compound, drug | MEM amino acid | Gibco | 11130–051 | |
| Chemical compound, drug | Glutamate | Sigma | G1251 | |
| Chemical compound, drug | Hydrochloric acid | Sigma | 320331 | |
| Chemical compound, drug | Low melt agarose | VWR | 97064–134 | |
| Chemical compound, drug | Sodium hydroxide | Sigma | 795429 | |
| Chemical compound, drug | Kanamycin sulfate | Sigma | 60615–5G | |
| Chemical compound, drug | Gentamicin sulfate | Sigma | 345814 | |
| Chemical compound, drug | Apramycin | Sigma | A2024-1G | |
| Chemical compound, drug | Streptomycin sulfate | Sigma | S9137 | |
| Chemical compound, drug | Tobramycin | Sigma | 614005 | |
| Chemical compound, drug | Trimethoprim | Sigma | T7883 | |
| Chemical compound, drug | Cyclohexamide | Sigma | C7698 | |
| Chemical compound, drug | Chloramphenicol | Sigma | C1919 | |
| Chemical compound, drug | Erythromycin | Sigma | E5389 | |
| Chemical compound, drug | Potassium chloride | Sigma | P9333 | |
| Chemical compound, drug | Magnesium chloride | Sigma | 63068 | |
| Chemical compound, drug | CCCP | Sigma | C2759 | |
| Chemical compound, drug | Oxyrase for broth | Sigma | SAE0013 | |
| Chemical compound, drug | Texas Red-X, Succinimidyl Ester | Thermo-Fischer | T20175 | |
| Chemical compound, drug | N,N-Dimethylformamide | Sigma | 227056 | |
| Chemical compound, drug | Glutathione | Sigma | G4251 | |
| Chemical compound, drug | Ascorbic acid | Sigma | A7506 | |
| Chemical compound, drug | Propidium iodide | Life-tech | P3566 | |
| Chemical compound, drug | Tetramethylrhodamine, methyl ester | Molecular Probes | T668 | |
| Chemical compound, drug | DL-Dithiothreitol | Sigma | D9779 | |
| Chemical compound, drug | Lysozyme from chicken egg white | Sigma | 62971–10 G-F | |
| Chemical compound, drug | Magnesium chloride hexahydrate | Sigma | 63068–250G | |
| Chemical compound, drug | Sucrose | Sigma | 84097–1 KG | |
| Chemical compound, drug | Water, Sterile. WFI Quality | Sigma | 4.86505.1000 | |
| Chemical compound, drug | Sodium deoxycholate | Sigma | 30970–25G | |
| Chemical compound, drug | Ammonium chloride | Sigma | 09718–250G | |
| Chemical compound, drug | DiOC6(3) | Sigma | 318426–250 MG | |
| Software, algorithm | MATLAB | https://www.mathworks.com/products/matlab.html | RRID:SCR_001622 | |
| Software, algorithm | NIS Elements | https://www.microscope.healthcare.nikon.com/products/software/nis-elements | RRID:SCR_002776 |