Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) |
C57BL/6J | CLEA, Japan, Inc | RRID:IMSR_JAX:000664 | WT mouse |
| Genetic reagent (Saccharomyces cerevisiae) |
RPN11-FLAGx3 | This paper | ||
| Strain, strain background (Escherichia coli) | BL21(DE3) | Sigma-Aldrich | Cat. #: 69450 | |
| Recombinant DNA reagent | pRK172-α-synuclein | PMID:8194594 | ||
| Recombinant DNA reagent | pRK172-α-synucleinΔC20 | PMID:30030380 | ||
| Antibody | anti-α-synuclein-pSer129 #64 (mouse monoclonal) | FUJIFILM Wako Chemicals | RRID:AB_2537218 | (1:1000) |
| Antibody | anti-α-synuclein 1–10 (rabbit polyclonal) | Cosmo Bio | RRID:AB_2860557 | (1:1000) |
| Antibody | anti-α-synuclein 131–140 (rabbit polyclonal) | Cosmo Bio | RRID:AB_2860558 | (1:1000) |
| Antibody | anti-α-synuclein 91–99 (mouse monoclonal) | BD Bioscience | RRID:AB_398108 | (1:1000) |
| Antibody | anti-α-synuclein-pSer129 (EP1646Y) (rabbit monoclonal) | Abcam | RRID:AB_869971 | (1:1000) |
| Antibody | anti-α-synuclein 115–122 (LB509) (mouse monoclonal) | Santa Cruz Biotechnology | RRID:AB_785898 | (1:200) |
| Antibody | anti-α-synuclein D37A6 (rabbit monoclonal) | Cell Signaling Technology | RRID:AB_1904156 | (1:1000) |
| Antibody | anti-α-synuclein-pSer129 (1175) (rabbit polyclonal) | PMID:23466394 | (1:1000) | |
| Antibody | anti-α-synuclein (Syn205) (mouse monoclonal) | Cell Signaling Technology | RRID:AB_490798 | (1:1000) |
| Antibody | anti-ubiquitin (rabbit polyclonal) | Proteintech | RRID:AB_671515 | (1:1000) |
| Antibody | anti-ubiquitin (rabbit polyclonal) | Dako | RRID:AB_2315524 | (1:10000) |
| Antibody | anti-GAPDH (mouse monoclonal) | Sigma-Aldrich | RRID:AB_2107445 | (1:1000) |
| Antibody | anti-FLAG M2 (mouse monoclonal) | Sigma-Aldrich | RRID:AB_262044 | (1:1000) |
| Antibody | anti-GFAP (D1F4Q) (rabbit monoclonal) | Cell Signaling Technology | RRID:AB_2631098 | (1:200) |
| Antibody | anti-Neurofilament-L (C28E10) (rabbit monoclonal) | Cell Signaling Technology | RRID:AB_823575 | (1:200) |
| Antibody | anti-NeuN (EPR12763) (rabbit monoclonal) | abcam | RRID:AB_2532109 | (1:1000) |
| Antibody | anti-tau (tauC) (rabbit polyclonal) |
PMID:26374846 | (1:200) | |
| Antibody | Goat anti-rabbit IgG, Alexa Fluor 488 | Thermo Fisher | RRID:AB_143165 | (1:1000) |
| Antibody | Goat anti-mouse IgG, Alexa Fluor 568 | Thermo Fisher | RRID:AB_2534072 | (1:1000) |
| Antibody | Goat anti-rabbit IgG, HRP | Thermo Fisher | RRID:AB_2536099 | (1:10000) |
| Antibody | Goat anti-mouse IgG, HRP | Thermo Fisher | RRID:AB_2536163 | (1:10000) |
| Antibody | Goat anti-rabbit IgG, biotin | Vector laboratories | RRID:AB_2313606 | (1:1000) |
| Software, algorithm | EZR | PMID:23208313 | ||
| Other | hematoxylin | Muto Pure Chemicals | Cat. #: 30002 | |
| Other | DAPI stain | Vector laboratories | Cat #: H-1500 |