Fig. 2. Characterization of JEV-ΔNS1.
a Comparison of growth kinetics between WT JEV (JEV-WT) and JEV-ΔNS1 virus in BHKNS1 cells. BHKNS1 cells were infected with either JEV-WT or JEV-ΔNS1 virus at an MOI of 0.1. The supernatants were harvested at the indicated time points and viral titers were determined as described above. Data represent the mean ± standard deviation (SD) of the triplicate measurements in a representative experiment. Statistical analysis was performed with unpaired t test and the asterisks denote statistical differences between the indicated groups. **p < 0.01; n.s. no statistical difference. b Western blotting analysis of purified JEV-WT and JEV-ΔNS1 viral particles. Following concentration and purification through PEG8000 precipitation and ultracentrifugation, equal amounts of purified WT and ΔNS1 viruses were subjected to Western blotting analysis using the specific anti-E monoclonal antibody or anti-C polyclonal antibody. All blots derived from the same experiment and were processed in parallel. c ELISA for the detection of the antibodies against both JEV and JEV-ΔNS1. Two independent experiments were performed in triplicate. Data represent the mean ± standard deviation (SD) of the triplicate measurements in a representative experiment. Statistical analysis was performed with unpaired t test. n.s. no statistical difference.