Fig. 6. Protective efficacy of one-shot immunization with JEV-ΔNS1 in C57BL/6 mice.
a Schematic diagram of animal experiment design and schedule. Four-week-old female C57BL/6 mice (n = 6 for each group) were i.p. immunized once with 1.4 × 107 IU of JEV-ΔNS1 and SA14-14-2 or 6 × 105 IU of YFV-17D. At 14 days after the immunization, the sera harvested from immunized mice was subjected to ELISA to detect the IgG antibody titers against JEV (b). ND not detected. c, d Viremia levels and weight changes in WT JEV challenged mice. All mice were i.p. injected with 3 × 107 PFU of WT JEV at 16 days post immunization and monitored for viremia (c), weight loss (d), and IgG post-challenge titers (e). The dashed lines in panels (b, c and e) represent the limits of detection. Data represent the mean ± standard deviation of six mice at each time point in each group. Statistical analysis was performed with unpaired t test and the asterisks denote statistical differences between the indicated groups. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; n.s. no statistical differences. Two independent experiments were performed, and data from one experiment are presented.