Fig. 4.

Gestational hypothyroidism renders offspring more susceptible to metabolic stress. Adult male offspring (16–18 weeks of age) were analysed. (a) Growth curve post initiation of feeding treatments showing increased weight gain in male offspring from hypothyroid mothers (n = 5–8 mice/group, two-way ANOVA; asterisks denote differences between LID-gest HFD and ND-gest HFD groups). (b) Animal weight at the end of feeding treatment (8 weeks) (n = 5–8 mice/group, one-way ANOVA). (c) Fasting blood glucose at the end of feeding treatment (8 weeks) (n = 5–8 mice/group, one-way ANOVA). (d) IPGTT (3 g/kg) (n = 5–8 mice/group, two-way ANOVA) and AUC analysis (n = 5–8 mice/group, one-way ANOVA). (e) Fasting basal insulin concentrations (n = 5–8 mice/group, one-way ANOVA). (f) ITT (0.75 U/kg) and AUC analysis (n = 5–8 mice/group, one-way ANOVA). (g) In vivo insulin responses to glucose (3 g/kg), (n = 8 mice/group, one-way ANOVA). *p < 0.05, **p < 0.01, ***p < 0.001 using the tests indicated above. Data are mean ± SEM. ND-gest ND, ND during gestation then ND; LID-gest ND, LID during gestation then ND; ND-gest HFD, ND during gestation then HFD; LID-gest HFD, LID during gestation then HFD