Fig. 6.

Effects of gestational hypothyroidism on glucose homeostasis in a second generation of animals. Adult MTg mice (8–10 weeks of age) were compared with age-matched controls. (a) Male weight at adult age (8–10 weeks) (n = 12 mice/group, Mann–Whitney U test). (b) Fasting blood glucose in males (n = 12 mice/group, Mann–Whitney). (c) IPGTT in males (3 g/kg) and AUC analysis (n = 12 mice/group, Mann–Whitney). (d) ITT in males (0.75 U/kg) and AUC analysis (n = 12 mice/group, Mann–Whitney). (e) Fasting insulin concentrations in males (n = 6 mice/group, Mann–Whitney). (f) In vivo insulin responses to glucose in males (3 g/kg), (n = 6 mice/group). (g) Female weight at adult age (8–10 weeks) (n = 12 mice/group, Mann–Whitney). (h) Fasting blood glucose in females (n = 12 mice/group, Mann–Whitney). (i) IPGTT in females (3 g/kg) and AUC analysis (n = 12 mice/group, Mann–Whitney). (j) ITT in females (0.75 U/kg) and AUC analysis (n = 12 mice/group, two-way ANOVA [left] and Mann–Whitney [right]). (k) Fasting insulin concentrations in females (n = 6 mice/group, Mann–Whitney). (l) In vivo insulin responses to glucose in females (3 g/kg), (n = 6 mice/group). (m) Quantification of islet area in males (n = 6 mice/group, Mann–Whitney). (n) Quantification of beta cell proliferation in males (n = 6 mice/group, Mann–Whitney). (o) Quantification of islet area in females (n = 6 mice/group, Mann–Whitney). (p) Quantification of beta cell proliferation in females (n = 6 mice/group, Mann–Whitney). *p < 0.05, **p < 0.01, ***p < 0.001 using the tests indicated above. Data are mean ± SEM. MTg, second generation of male (in orange) and female (in cyan) offspring from males born to hypothyroid mothers