FIGURE 2.

Protein disulfide isomerase (PDI) disaggregase activity is effective at sub-stoichiometric molar ratios. To initiate fibril formation at 0 h, α-synuclein mutant E46K was diluted to 70 μM at 37°C with constant agitation. Data represent the averages ± standard deviations of three independent experiments. (A) PDI was mixed at the indicated molar ratios with α-synuclein at 0 h. ThT fluorescence at 482 nm was recorded 30 h (gray bars) or 52 h (black bars) after the initiation of fibrillization. (B) Samples of α-synuclein were allowed to aggregate for 30 h. After recording ThT fluorescence at 30 h (gray bars), the indicated molar ratios of PDI were added to the sample for another 22 h before a second ThT measurement was taken (black bars).