Figure 3.

Effects of proteasome or CRL inhibitors on SAMHD1 accumulation in HCMV-infected cells. HF cells in six-well plates were mock-infected or infected with different HCMV strains [Towne (A), Toledo (B), or JHC (C)] at an MOI of 1. Cells were harvested at the indicated time points. For inhibitor treatment, cells were treated with DMSO, MG132 (5 μM), or MLN4924 (1 μM) at 72 h post infection and incubated for another 24 h. Total cell lysates were prepared, subjected to SDS-8% PAGE, and immunoblotted with antibodies for SAMHD1, IE1/IE2, pp28 (UL99), and β-actin. The SAMHD1 and non-specific bands are indicated as arrowheads and open circles, respectively.