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. 2020 Jul 30;10:391. doi: 10.3389/fcimb.2020.00391

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Copyright © 2020 Hyeon, Lee, Kim, Lee and Ahn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of SAMHD1 in viral replication compartments. HF cells in chamber slides were infected with HCMV (Towne) at an MOI of 1 for 90 h and double-label confocal IFA was performed with anti-SAMHD1 and anti-UL112-113 p84 antibodies. FITC-labeled anti-rabbit IgG and rhodamine/Red X-coupled anti-mouse IgG were used for visualization. Hoechst stain was used to stain cell nuclei. Three single-labeled images and a merged image for SAMHD1 and UL112-113 are shown. The top and middle images were selected to include some cells showing UL112-113 foci, while the bottom images show cells with fully grown viral replication compartments.