FIGURE 5.

Effect of apocynin on reducing major organ (liver, heart, and brain) oxidative stress associated with dietary obesity and accelerated aging in mice. Apocynin was supplied in drinking water during high‐fat diet (HFD) period. A, Changes in bodyweight. B, Apocynin detected in tissue homogenates by HPLC‐MS/MS after treatment. C‐E, The levels of ${\mathrm{O}}_2^{·‐}$ production in tissue homogenates detected by lucigenin‐chemiluminescence. Left panels: Representative kinetic detection of ${\mathrm{O}}_2^{·‐}$ production. Right panels: Statistical analysis. Data were presented as % of NCD (100%). n = 6 mice/group. *P < .05 for indicated values vs NCD values. †P < .05 for indicated values vs HFD values. HPLC‐MS/MS, HPLC coupled to a quadrupole‐linear ion‐trap tandem mass spectrometry; NCD, normal chow diet