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. 2020 Jul 30;8:669. doi: 10.3389/fcell.2020.00669

TABLE 1.

MiRNAs identified in MS2-tagged RNA affinity assay.

miRNA MS2-C MS2-F8 Fold change Fold change Log2
hsa-miR-92a-3p 1863 7696 4.13 2.05
hsa-miR-19b-3p 516 6122 11.86 3.57
hsa-miR-17-5p 217 4907 22.61 4.5
hsa-miR-25-3p 800 4244 5.31 2.41
hsa-miR-191-5p 286 3012 10.53 3.4
hsa-miR-19a-3p 282 2736 9.7 3.28
hsa-miR-93-5p 189 1845 9.76 3.29
hsa-miR-222-3p 154 845 5.49 2.46
hsa-miR-7-5p 63 673 10.68 3.42
hsa-miR-24-3p 74 662 8.95 3.16
hsa-miR-106a-5p 27 588 21.78 4.44
hsa-miR-221-3p 70 524 7.49 2.9
hsa-miR-125a-5p 97 522 5.38 2.43
hsa-miR-196a-5p 92 375 4.08 2.03
hsa-miR-186-5p 35 355 10.14 3.34
hsa-miR-196b-5p 46 328 7.13 2.83
hsa-miR-130b-3p 20 293 14.65 3.87
hsa-miR-3607-3p 1 278 278 8.12
hsa-miR-16-5p 10 255 25.5 4.67
hsa-let-7f-5p 55 250 4.55 2.18
hsa-miR-378a-3p 10 235 23.5 4.55
hsa-let-7g-5p 16 214 13.38 3.74
hsa-miR-454-3p 30 207 6.9 2.79
hsa-miR-425-5p 48 203 4.23 2.08
hsa-miR-301a-3p 15 182 12.13 3.6
hsa-miR-103a-3p 12 181 15.08 3.91
hsa-miR-505-3p 39 172 4.41 2.14
hsa-miR-18a-5p 2 167 83.5 6.38
hsa-miR-20a-5p 11 163 14.82 3.89
hsa-miR-5701 1 154 154 7.27
hsa-miR-7641 30 139 4.63 2.21
hsa-miR-424-5p 21 122 5.81 2.54
hsa-miR-1275 12 117 9.75 3.29
hsa-miR-1307-3p 19 97 5.11 2.35
hsa-miR-151a-5p 14 89 6.36 2.67
hsa-miR-185-5p 7 87 12.43 3.64
hsa-miR-378a-5p 19 85 4.47 2.16
hsa-miR-30c-5p 19 83 4.37 2.13
hsa-miR-455-3p 10 82 8.2 3.04
hsa-miR-31-5p 6 81 13.5 3.75
hsa-miR-324-5p 4 81 20.25 4.34
hsa-miR-106b-3p 11 78 7.09 2.83
hsa-miR-29a-3p 5 72 14.4 3.85
hsa-miR-664b-3p 1 69 69 6.11
hsa-miR-3651 2 68 34 5.09
hsa-miR-10a-5p 14 63 4.5 2.17
hsa-miR-532-5p 14 62 4.43 2.15
hsa-miR-34a-5p 7 57 8.14 3.03
hsa-miR-93-3p 10 49 4.9 2.29
hsa-miR-1246 10 48 4.8 2.26
hsa-miR-149-5p 7 47 6.71 2.75
hsa-miR-29c-3p 1 46 46 5.52
hsa-miR-1269b 8 45 5.63 2.49
hsa-miR-148b-3p 2 41 20.5 4.36
hsa-miR-15b-5p 2 41 20.5 4.36
hsa-miR-361-5p 4 38 9.5 3.25
hsa-miR-128-3p 6 37 6.17 2.62
hsa-miR-3653-3p 1 36 36 5.17
hsa-miR-320a 6 34 5.67 2.5
hsa-miR-194-5p 6 33 5.5 2.46
hsa-miR-324-3p 3 33 11 3.46
hsa-miR-421 7 32 4.57 2.19
hsa-miR-30b-5p 1 31 31 4.95
hsa-miR-146b-5p 5 30 6 2.58
hsa-miR-374b-5p 37 72 1.95 0.96
hsa-miR-144-5p 0 0

The list of 64 miRNAs detected in MS2-F8 3′UTR (MS-F8) sample with the highest fold change compared to control (MS2-E) with log2(fold change) >2 and read counts ≥30 (except miR-374b and miR-144). Bold miRNAs were predicted by prediction algorithms. The predicted miRNAs and their association scores are summarized in Supplementary Table S1. The underlined miRNAs were selected for further evaluation. Co-data for control miRNA:miR-144-5p and miR-374b-3p included for comparison.