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. 2020 Aug 5;10:13167. doi: 10.1038/s41598-020-69897-w

Figure 1.

Figure 1

Feasibility of CD8+ T cell staining with dual-fluorochrome-encoded pB7.2 tetramers. (A) B7tg mice were inoculated i.n. with sublethal dose of VACV, and, after 4 weeks, challenged i.n. with a lethal dose of the virus (see “Materials and methods”). Splenocytes from infected mice were stained with a single fluorochrome-labelled p/B7.2 tetramer (topmost row) or 10 possible two-colour combinations of the B8R70–78/B7.2 tetramers in a single staining reaction (lower panels). (B) A representative binary encoding strategy querying 10 different specificities in a single reaction using VACV-reactive splenocytes elicited in the experiment described in (A) Red, positive VACV pB7.2 tetramer staining; blue, no staining with VACV pB7.2 tetramer; green, no staining with self p/B7.2 tetramers. See Figures S1, S2 for additional binary encoding description and data for tracking 10 distinct T cell specificities in a single pot.