Figure 4.

ECTV-reactive CD8⁺ T cells does not recognise the C4R_70–78 peptide, the ortholog of B8R_70–78. (A) B7.2^tg mice were inoculated i.n. with VACV as in Fig. 1. Spleens were harvested from mock (n = 3), ECTV (n = 3) or VACV (n = 3) inoculated mice 8–10 days after secondary infection. CD8⁺ T cell responses were monitored using pB7.2 tetramers using dual color labelling and multi-dimensional encoding strategy as in Fig. 1. Representative contour plots depicting CD8⁺ T cell response to B8R_70–78 and C4R_70–78 in ECTV- and VACV- inoculated splenocytes. CD8⁺ T cell response to common epitope D1R_808-817, served as positive control. (B) Frequencies (% of CD8⁺) of B8R_70–78, C4R_70–78 or D1R_808-817 (positive control) reactive CD8⁺ T cells in VACV-specific CD8⁺ T cells defined by pB7.2 tetramer staining in infected splenocytes. Data are mean ± sem; n as in (A).