Table 1.

Previously reported VACV-derived CD8⁺ T cell epitopes presented by B7.2: their homology to ECTV proteome and recognition by CD8⁺ T cells.

VACV ORFsa	VACV epitopesb	ECTVc	Recognition by
			VACV-reactive T cellsb,d	ECTV-reactive T cellse	hu PBMCsf
D1R808–817	RPSTRNFFEL	•	+	+	+
A34R82–90	LPRPDTRHL	•	+	+	–
D1R686–694	HPRHYATVM	•	+	+	+
J6R303–311	MPAYIRNTL	•	+	+	+
F4L6–14	APNPNRFVI	•	+	+	–
E2L216–224	RPRDAIRFL	•	+	+	+
A3L192–200	SPSNHHILL	•	+	+	+
D5R375–383	LPKEYSSEL	•	+	+	–
L4R37–45	FPRSMLSIF	•	+	+	+
E9L526–534	FPYEGGKVF	•	+	+	+
I6L282–291	IPKKIVSLL	•	–	+	–
B15R91-101	IPDEQKTIIGL	•	–	+	–
D9R26-35	IPRSKDTHVF	•	–	+	–
A24R1002-1010	KPYASKVFF	•	–	+	–
B8R70–78 g	FPKNDFVSF	K72N	+	–	+
N2L104–113	RPNQHHTIDL	Q107K	+	–	+
O1L335–344	RPMSLRSTII	NSH	+	–	+
A4L126–135	APASSLLPAL	NSH	–	–	+
B22R72-80	TVADVRHCL	NSH	–	–	+
E9L175–183	FPSVFINPI	•	–	–	+
A11R22–30	YPSNKNYEI	•	–	nt	+
A20R162–170	IPKYLEIEI	•	–	nt	+
C19L/B25R78–86	NPSVLKILL	N78K	–	nt	+
E5R131-1–40	NPSKMVYALL	•	–	nt	+
G2R140-149	VPITGSKLIL	NSH	–	nt	+
I6L237–245	FPTNTLTSI	•	+	nt	–
I6L282–291	LPSNVEIKAI	•	–	nt	+

^aOpen reading frames and location of epitopes defined based on Copenhagen reference strain (VACCC, ID 10,249).

^bVACV-derived epitopes recognised by CD8⁺ T cells reported by us and others in mice and humans²⁰.

^cAmino acid changes in the orthologous ECTV peptide; •, conserved sequences with 100% homology; NSH, no significant homology; based on Netblast (blastcl3: www.ncbi.nlm.nih.gov) using ECTV txid12643.

^dB7.2^tg mouse CD8⁺ T cells that react to VACV²⁰.

^eB7.2^tg mouse CD8⁺ T cells that react to ECTV (this study): + , positive response (see Fig. 2); –, no response; nt, not tested.

^fhu, VACV-reactive CD8⁺ T cells in human PBMC²⁰.

^gB8R orthologue in ECTV is C4R based on Netblast using ECTV txid12643.