Figure 1.

Summary of the trial design and inclusion of dogs. A total of 35 dogs were enrolled and 20 dogs had to be excluded for various reasons, including unscheduled visits, non-compliance with the dosage, progressive weight loss and diarrhea, receiving prior treatment with steroids, cyclosporine, and/or antibiotics. Each box represents the period in which the dogs received Purina HA diet (gray), treatment 1 (hydrolyzed diet supplemented with PRB+GAG; black) and treatment 2 (hydrolyzed diet only; upward diagonal). Blood samples for metabolomics analysis were collected during the initial physical examination (pre-treatment, visit 1, day 0), during a subsequent visit (visit 2, day 14 ± 2), and after the treatment schedule (post-treatment, visit 3, day 70 ± 2).