FIGURE 1.

Effect of LGG conditioned medium on macrophage and E. coli viability. (A) Macrophages were grown in 24 well plates and exposed to LPS, LGG conditioned medium (LGG-CM), or E. coli cells for 6 h (olive), or 24 h (light green). Cell viability was assessed by MTT reduction. LPS concentration was 20 μg/ml; LGG conditioned medium was 10% or 100%. Control was no additions. (B) Viability of E. coli HfrC after incubation with LGG supernatant for 6 h (light blue) and 24 h (orange). Recovered colony forming units (CFU) were determined. Control cells had no treatment and CFU numbers were set 100%. All data shows means ± SD; experiments were performed in triplicate and repeated 3 times. One-way ANOVA showed no significant decrease in viability between any of the different conditions.