FIGURE 4.

Role of NO and ROS inhibitor combination in bacterial digestion in the presence of LGG-CM. The effect of iNOS + NADPH oxidase inhibitors on bacterial digestion were investigated in the presence of LGG-CM. The inhibitors used were Apocyanin (Apo) = NADPH oxidase inhibitor (A); Cell permeable variants of SOD and Catalase that scavenge reactive oxygen species (B). LNMMA = iNOS inhibitor (C). A combination of LNMMA and Apocyanin were used in (D). Experiments were performed in duplicate and repeated three times. Error bars indicate ± standard error of the mean. Statistical analysis was carried out using Graph Pad Prism 3. The Dunnett’s multiple comparison post-hoc tests was performed after observing a significant difference from One way ANOVA. An asterisk (*) indicates significant difference (p < 0.01) between the treatments and control.