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. 2020 Jul 30;11:1173. doi: 10.3389/fphar.2020.01173

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Copyright © 2020 Yu, Song, Cao, Li, Zhao, Chen, Li, Chen, Yu and Xu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HE suppressed the apoptosis of neurons after CI/R injury. Mice were treated with HE (106 μmol/kg) or vehicle (i.p.) once daily for 3 consecutive days after MCAO. Western blot was used to detect the expression levels of apoptosis-related proteins, and TUNEL staining was used to measure the apoptotic levels after CI/R injury. (A) Representative images show the expression levels of Bcl-2, Bax by Western Blot analysis with β-actin as a loading control. (B) Quantitative analysis of the Bcl-2 to Bax ratio. (C) TUNEL-positive cells showed green fluorescence, and DAPI-positive cells showed blue fluorescence. (D) Quantification of the number of TUNEL-positive neural cells in each group. HE treatment significantly decreased the number of TUNEL-positive neural cells after CI/R injury. Scale bar, 25 µm; magnification, 20x. The bar graphs represent the mean ± SEM of three brains in each group. *P < 0.05 versus the CI/R group.