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. 2020 Jul 30;11:1173. doi: 10.3389/fphar.2020.01173

Figure 6.

Figure 6

HE alleviated CI/R injury through inhibiting the MEK4/7/JNK axis. Mice were treated with HE (106 μmol/kg, i.p.) or vehicle once daily for 3 consecutive days after ischaemia. After 60 min of ischaemia and 3 days of reperfusion, cerebral tissue was collected, and protein levels were detected by Western blot. (A) The representative images show the levels of p-p38, p38, p-ERK, ERK, p-JNK, JNK, and β-tubulin. β-tubulin was used as a loading control. (B) Quantitative analysis showed that HE decreased the expression level of p-JNK, but not that of p-ERK or p-p38, after CI/R injury. (C) Representative images show the levels of p-MKK4, MKK4, p-MKK7, MKK7, and β-tubulin. β-tubulin was used as a loading control. (D) Quantitative analysis showed that HE decreased the expression levels of p-MKK4 and p-MKK7 after CI/R injury. The bar graphs represent the mean ± SEM of three brains in each group. *P < 0.05 versus the CI/R group.