Fig. 1.

NBMI treatment does not affect survival or lifespan in C. elegans. Approximately 1000 worms were treated with NBMI (0, 10, 100, or 1000 μM) for different time periods, followed by death rate analysis 24 h post-treatment. For lifespan assay, 60 worms per group were picked each day into a new OP50 seeded plate. (a) Death rate of wild type N2 worms treated with 0-1000 μM NBMI. (b) Death rate of bus-5 mutant worms treated with 0-1000 μM NBMI. (c) Lifespan of worms with life-time treatment of NBMI (0, 10, 100, and 500 μM). In (a) and (b), data were analyzed with Chi-square test, n = 3. In (c), data represent one of the three independent experiments. Comparisons of the survival curves were made with Log-rank (Mantel-Cox) test.