Fig. 2.

NBMI treatment reduces brood size of C. elegans on adult day 1. Synchronized L1, L3, or L4 stage worms were treated with 0-1000 μM NBMI for 24 h, and the egg numbers were counted. The total number of broods was calculated from adult day-1 to adult day-5. (a-c) The brood size of wild type N2 worms treated with NBMI at L1 stage (a), L3 stage (b), or L4 stage (c). Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test, n = 3. The horizontal bars represent a statistical significant difference between the groups with post-hoc multi-comparison test. *P < .05, **P < .01, and ***P < .001.