Worms treated with NBMI show significant developmental delay. Synchronized L1 stage worms were treated with 0-1000 μM NBMI for 24 h, followed by assessment of developmental stage 24 h post-treatment. Worms in the 0 μM NBMI group received 0.1 % DMSO as vehicle control. *P < .05, **P < .01, and ***P < .001 as compared with untreated control. #P < .05, ##P < .01, and ###P < .001 as compared with the 10 μM NBMI group. The horizontal bars represent a statistical significant difference between the groups with post-hoc multi-comparison test. *P < .05, **P < .01, and ***P < .001 (Chi-square test, n = 3 per treatment group).