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. 2020 Jul 31;7:930–937. doi: 10.1016/j.toxrep.2020.07.012

Fig. 5.

Fig. 5

NBMI treatment induces nuclear translocation of DAF-16. DAF-16 nuclear localization (cytosol (a), intermediate (b) and nuclear (c)) was observed following NBMI treatment for 24 h in the TJ356 strain [daf-16p::daf-16a/b::GFP + rol-6(su1006)]. Worms in the 0 μM NBMI group received 0.1 % DMSO as vehicle control. (d) Quantification of DAF-16 nuclear localization as described in the method section (Chi-square test, n = 3). White arrowheads indicate the DAF-16::GFP puncta in the nuclear region. Scale bar = 50 μM. *P < .05, **P < .01, and ***P < .001 as compared with untreated control. #P < .05, ##P < .01, and ###P < .001 as compared with the 100 μM NBMI group.