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. Author manuscript; available in PMC: 2020 Aug 6.
Published in final edited form as: Brain Res Bull. 2019 Nov 13;154:102–105. doi: 10.1016/j.brainresbull.2019.11.005

Fig. 1. Minocycline treatments blocked bilirubin-induced expressions of TNFα and iNOS in rat cerebellar glia.

Fig. 1.

Mixed glial cells were pretreated with or without minocycline (10 μM, 2h) followed by bilirubin (1 μM) treatments for additional 24 h (BR + M vs. BR). The lysates were immunoblotted with anti-iNOS and TNFα antibodies. The anti-β-actin antibody was used to confirm an equal amount of protein loading in each gel lane. All cultures were treated in triplicate at the indicated times and data are represented as the mean ± SEM (### p < 0.001, treated with bilirubin vs. control; ** p < 0.01, treated with minocycline and bilirubin vs. bilirubin only by Student’s t-test). This experiment was repeated three times.