Figure 5.

Western blot analysis confirmed that transfection with the recombinant AFP lentivirus increased the AFP expression in the subcutaneous hepatocellular carcinoma xenografts inoculated in BALB/c nude male mice. Compared to the control group (xenografts transfected with negative control lentivirus), overexpression of AFP increased the protein expression of PD-L1, B7-H4, and P65. There were no differences in B7-H3 protein expression between the AFP and control groups (^∗∗ indicates P < 0.01 and ^∗∗∗ indicates P < 0.001).