FIG 6.

Germination and outgrowth timeline. (A) Morphological changes during spore germination and outgrowth were determined by microscopic analysis. Samples were harvested at different time points during spore revival. (B) Changes in spore proteins were analyzed relative to metabolically ¹⁵N-labeled vegetative cells. This approach was used to study the spore germination and outgrowth timeline unless otherwise stated. (C) Amino acid recycling throughout spore revival was monitored by germinating ¹⁵N-labeled spore in ¹⁴N-minimal medium. (D) Incorporation of SILAC in protein, an indication of protein synthesis, was monitored during early and later stages of germination and outgrowth in ¹⁴N spores.