FIG 3.

Changes in fluorescence intensity of young, MOPS medium-grown B. subtilis PdhD-IpHluorin spores during germination. The fluorescence intensity of spores from germinating day 2 (n = 87) PdhD-IpHluorin spores prepared in MOPS liquid medium was measured over 80 min after initiation of germination. The fluorescence intensity of the wild-type spores was subtracted from the PdhD-IpHluorin fluorescence intensity to eliminate the background fluorescence of the spore itself. The subtracted value was 2.3% of the zero time value.