FIG 4.

Fluorescence images of germinating young B. subtilis PdhD-IpHluorin spores prepared in MOPS medium. Shown is a collage of images of an individual germinating spore captured at different time points. The images were captured every 5 min using two different channels: PH3 (phase contrast) and GFP (IpHluorin). The PH3 channel visualizes the germination over time (phase bright to phase dark). The GFP channel visualizes changes in IpHluorin fluorescence intensity over time. The fluorescent foci are indicated by yellow arrows.