TABLE 1.
Spore sample (B. subtilis AR71) | No. of spores analyzed |
% of phase-bright spores with 0 to 3 foci |
||||
---|---|---|---|---|---|---|
R1 | R2 | 0 | 1 | 2 | 3 | |
MOPS | ||||||
D2 | 37 | 48 | 29 | 41 | 27 | 3 |
D4 | 71 | 52 | 24 | 38 | 34 | 4 |
2× SG | ||||||
D2 | 49 | 18 | 39 | 23 | 20 | |
D4 | 69 | 67 | 13 | 15 | 6 |
Numbers of MalS-GFP fluorescent foci measured in the dormant (time zero) spores prepared in MOPS and 2× SG medium are presented. For spores prepared in MOPS, the numbers represent averages of results from two biological replicates (R1 and R2). D2 (day 2) and D4 (day 4) represent the age of dormant spores.