FIG 4.

A large conserved plasmid carrying antibiotic resistance is found in ST1193 isolates. (A) Alignment of plasmids pSCU-390-1, pSCU-204-1, pNMEC-075A (GenBank accession no. CP030112.1), and pC32_1 (GenBank accession no. CP041619.1) using BRIG (BLAST Ring Image Generator) (50); numbering starts at the F1B replicon. Select genetic regions shared by all of the plasmids are indicated on the outside ring, including plasmid replicons identified by Plasmid Finder. Antibiotic resistance genes in plasmid pSCU-390-1 are annotated in the inner ring. The variable region from approximately 40 to 60 kb, indicated by gaps in the alignment, is shown in panel B. (B) Comparison of the variable regions located between 40 and 60 kb in the conserved ST1193 plasmids. Transposable elements and resistance genes were identified using ResFinder (48) and GAMR (24) and visualized using BioRender. IS elements are indicated by light gray boxes, with their name above the box; transposons and the class 1 integron are indicated by dark gray boxes, with their name above. Conserved inverted repeats known to be associated with transposable element boundaries are indicated by triangles above the boundaries. Dashed lines indicate breakpoints (defined by sequence alignment) of interrupted elements; partial elements are indicated by a prime symbol following their name. Antibiotic resistance genes are indicated by black arrows, with their name underneath.