FIG 7.

EhCoactosin S147A and S147D do not affect E. histolytica adherence or phagocytosis. (A) Adherence to fixed Chinese hamster ovary (CHO) cells. To enable combined analysis of multiple experiments, the number of adherent trophozoites for each cell line was expressed as the percentage of adherent empty vector control cells adherent on that day (n = 6 independent experiments). Lines indicate means and SDs. There was considerable variability from day to day. Expression of the Coac-WT, Coac-A, and Coac-D variant EhCoactosin proteins had no significant effect. Phagocytosis of healthy (B) and apoptotic (C) Jurkat T lymphocytes. Phagocytosis of fluorescently labeled Jurkat cells was quantified for each trophozoite line using flow cytometry, and the phagocytic index was calculated as the percentage of phagocytic amoebae × the mean fluorescence index of phagocytic amoebae. See Fig. S1 in the supplemental material for the flow cytometry gating scheme. The means from multiple readings in three independent experiments were expressed as the percentage of phagocytic index for empty vector control cells to enable combined statistical analysis for experiments performed on multiple days. Each data point indicates the average normalized phagocytic index for 1 day (n = 3 for each mutant). Expression of the Coac-WT, Coac-A, and Coac-D variant EhCoactosin proteins had no significant effect.