FIG 5.

Sll0067 promotes the disappearance of free MG and GSH. (A) Schematic representation of the conjugation of GSH with MG to produce hemithioacetal, a reaction always regarded as spontaneous. (B and C) Influence of Sll0067, before or after heat inactivation, on the kinetics of the disappearance of MG and GSH measured at 30°C in phosphate buffer containing 50 μM MG, 1,000 μM GSH, and 2 μM Sll0067 GST. (D) Decrease in GSH and MG measured in the absence or presence of Sll0067 before or after heat inactivation. Data, expressed as μM mg⁻¹ of protein s⁻¹, represent means ± the SD of three experiments. *, Significant difference between the decreases of GSH and MG observed in the presence or absence of Sll0067 activity (t test, P < 0.05).