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Kinetic parameters of Sll0067 activity on both MG and GSH substrates
| Parameter | Mean ± SDa
|
|
|---|---|---|
| MG | GSH | |
| kcat (s−1) | NA | 0.15 ± 0.02 |
| Km (μM) | NA | 421.70 ± 70.50 |
| kcat/Km (M−1 s−1) | NA | 355.60 ± 0.12 |
| K0.5 (μM) | 221.7 ± 26.3 | NA |
| nh | 1.873 ± 0.261 | NA |
Enzymatic activities were measured as described in Materials and Methods using various concentrations of the following substrates MG and GSH. The results are presented as the means of three independent measurements. NA, not applicable.
