FIG 9.

ZIKV-infected mouse brain displays local BBB impairment, leukocyte infiltration, and CAM upregulation. (a) RT-qPCR analyses of ZIKV genome in the brain of ZIKV-infected Ifnar^−/− mice 7 dpi. (b) Picture of dissected brains from EB-injected mock- and ZIKV-infected mice at 7 dpi. (c) Quantification Evans blue fluorescence in brain slices from mock- and ZIKV-infected animals. Results are expressed means ± SEMs (n = 3) and analyzed using a Wilcoxon-Mann-Whitney test. ****, P < 0.0001 compared to CT. (d) Three-micron consecutive paraffin sections were processed with Luxol blue and stained either with an anti-pan-flavivirus or an anti-CD45 (lymphoid cells) (brown labeling) antibody. Bars. 10 μm. (e) RT-qPCR analyses of inflammatory genes in ZIKV-infected brains. Results are expressed as means of the fold change (n = 3) using GAPDH as a housekeeping gene (genes where the ratio gene/housekeeping gene is statistically significant [P < 0.05] from CT) (see Fig. S8a). (f) RT-qPCR analyses of CAMs in ZIKV-infected brains. Results are expressed as means of the fold change (n = 3) using GAPDH as a housekeeping gene (genes where the ratio gene/housekeeping gene is statistically significant [P < 0.05] from CT) (see Fig. S8a). (g) RT-qPCR analyses of TJ and AJ genes in ZIKV-infected brains. Results are expressed as means of the fold change (n = 3) using GAPDH as a housekeeping gene (genes where the ratio gene/housekeeping gene is statistically significant [P < 0.05] from CT) (see Fig. S8a).