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. 2020 Jul 10;9(7):1661. doi: 10.3390/cells9071661

Figure 3.

Figure 3

Rescue experiments demonstrate involvement of the Bcl-2 family members in cell death elicited by the combination treatment of gamitrinib and HDAC inhibitors. (A) U87 and T98G cells were treated with gamitrinib, panobinostat or the combination of both for 24h. Whole cell lysates were subjected for protein capillary electrophoresis. Vinculin serves as a loading control; (B) GBM14 and GBM43 cells were treated with gamitrinib, romidepsin or the combination of both for 24h. Whole cell lysates were subjected to standard western blotting. Actin is a loading control; (C) T98G cells were transfected with non-targeting (NT) siRNA, BAK, Noxa or BAX specific siRNAs. Following transfection, the cells were exposed to vehicle, gamitrinib (GTPP), panobinostat or the combination treatment of gamitrinib and panobinostat for 24h. Thereafter, cells were labeled with propidium iodide (PI) dye and analyzed by flow cytometry (n = 3). Shown are means and SD. (D) Shown are protein capillary electrophoresis analyses of T98G cells transfected with the indicated siRNAs. ANOVA was used for statistical analysis. **** p < 0.001.