Table 3.
This table shows the lipid classes detected with this LC–MS lipidomics method. The number of species per lipid class and the measured adducts (protonated: [M+H]+, deprotonated: [M-H]−, protonated with water loss: [M+H-H2O]+, sodiated: [M+Na]+, potasiated: [M+K]+, ammoniated: [M+NH4]+) are also shown. The internal standard used for semi-quantification are also shown: butyryl-d7-L-carnitine (abbreviated to IS_Car_4:0-d7), N-tetradecylphosphocholine-d42 (abbreviated to IS_LPC_14:0-d42), hexadecanoyl-L-carnitine-d3 (abbreviated to IS_Car_16:0-d3), heptadecanoic-d33 acid (abbreviated to IS_FA_17:0-d33), 1,2-dimyristoyl-d54-sn-glycero-3-[phospho-L-serine] (abbreviated to IS_PS_28:0-d54), 1-palmitoyl-d31-2-oleoyl-sn-glycero-3-phosphoinositol (abbreviated to IS_PI_34:1-d31), N-palmitoyl-d31-D-erythro-sphingosylphosphorylcholine (abbreviated to IS_SM_34:1-d31), 1-palmitoyl-d31-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (abbreviated to IS_PG_34:1-d31), 1-palmitoyl-d31-2-oleoyl-sn-glycero-3-phosphate (abbreviated to IS_PA_34:1-d31), N-palmitoyl-d31-D-erythro-sphingosine (abbreviated to IS_Cer_16:0-d31), 1-palmitoyl-d31-2-oleoyl-sn-glycero-3-phosphocholine (abbreviated to IS_PC_34:1-d31), 1-palmitoyl-d31-2-oleoyl-sn-glycero-3-phosphoethanolamine (abbreviated to IS_PE_34:1-d31), glyceryl tri(pentadecanoate-d29) (abbreviated to IS_TG_45:0-d87).
| Analyte Class | No. of Species | Adducts | Internal Standard |
|---|---|---|---|
| Acyl-carnitines | 48 | [M+H]+ | IS_Car_4:0-d7, IS_Car_16:0-d3 |
| Ceramides | 85 | [M+H]+, [M+H-H2O]+ | IS_Cer_16:0-d31 |
| Cardiolipins | 56 | [M-H]− | IS_TG_45:0-d87 |
| Diacylglycerols | 6 | [M+H-H2O]+, [M+Na]+, [M+K]+ | IS_TG_45:0-d87 |
| Gangliosides (GM1) | 24 | [M-H]− | IS_PG_34:1-d31 |
| Hexosylceramides | 56 | [M+H]+, [M+H-H2O]+ | IS_Cer_16:0-d31 |
| Lyso-phosphatidylcholines | 23 | [M+H]+ | IS_LPC_14:0-d42 |
| Lyso-phosphatidyethanolamines | 19 | [M+H]+ | IS_LPC_14:0-d42 |
| Lyso-phosphatidylinositols | 19 | [M-H]− | IS_PI_34:1-d31 |
| Lyso-phosphoserines | 20 | [M-H]− | IS_PS_28:0-d54 |
| Lyso-cardiolipins | 23 | [M-H]− | IS_TG_45:0-d87 |
| Monoacylglycerols | 1 | [M+H-H2O]+, [M+Na]+, [M+K]+ | IS_TG_45:0-d87 |
| Phosphatidic acids | 26 | [M-H]− | IS_PA_34:1-d31 |
| Phosphatidylcholines | 43 | [M+H]+ | IS_PC_34:1-d31 |
| Phosphatidylethanolamines | 19 | [M+H]+ | IS_PE_34:1-d31 |
| Phosphatidylglycerol | 34 | [M-H]− | IS_PG_34:1-d31 |
| Phosphatidylinositols | 21 | [M-H]− | IS_PI_34:1-d31 |
| Phosphatidylserines | 36 | [M-H]− | IS_PS_28:0-d54 |
| Sulfatides | 72 | [M-H]− | IS_PG_34:1-d31 |
| Sphingomyelins | 54 | [M+H]+, [M+Na]+, [M+K]+ | IS_SM_34:1-d31 |
| Triacylglycerides | 89 | [M+H]+, [M+NH4]+, [M+Na]+, [M+K]+ | IS_TG_45:0-d87 |