Figure 1.
Anti-influenza virus activity of compounds 6, 7, and 13, in MDCK cells at day 3 p.i. with A/PR/8/34 virus. (A) Representative images showing complete inhibition of viral cytopathic effect (CPE) at compound concentrations devoid of any cytotoxicity. (B) Reduction in virus yield, as determined by RT-qPCR for viral RNA in the supernatant (lower limit of detection: 101.6 copies). Curve fitting by GraphPad Prism, on two data points from one experiment performed in duplicate. Full grey line: Virus yield for untreated virus control; red dashed line: 100-fold reduction in virus yield. (C) Compound cytotoxicity in mock-infected cells, determined by MTS cell viability assay (mean data from three experiments). Full grey line: 100% viability in the cell control receiving no compound; red dashed line: 50% cell viability.